Titelaufnahme

Titel
Enzymatic synthesis of hyaluronic acid vinyl esters for two-photon microfabrication of biocompatible and biodegradable hydrogel constructs
VerfasserQin, Xiao-Hua ; Gruber, Peter ; Markovic, Marica ; Plochberger, Birgit ; Klotzsch, Enrico ; Stampfl, Jürgen In der Gemeinsamen Normdatei der DNB nachschlagen ; Ovsianikov, Aleksandr In der Gemeinsamen Normdatei der DNB nachschlagen ; Liska, Robert In der Gemeinsamen Normdatei der DNB nachschlagen
Erschienen in
Polymer Chemistry, 2014, Jg. 5, H. 22, S. 6523-6533
Erschienen2014
Ausgabe
Published version
SpracheEnglisch
DokumenttypAufsatz in einer Zeitschrift
URNurn:nbn:at:at-ubtuw:3-1115 Persistent Identifier (URN)
DOI10.1039/c4py00792a 
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Enzymatic synthesis of hyaluronic acid vinyl esters for two-photon microfabrication of biocompatible and biodegradable hydrogel constructs [0.97 mb]
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Zusammenfassung (Englisch)

Two-photon polymerization (2PP) allows 3D microfabrication of biomaterial scaffolds with user-defined geometry. This technique is highly promising for 3D cell culture and tissue engineering. However, biological applications of 2PP require photopolymerizable hydrogels with high reactivity and low cytotoxicity. This paper describes a novel hydrogel system based on hyaluronic acid vinyl esters (HA-VE), which enabled fast 2PP-fabrication of 3D hydrogel constructs with m-scale accuracy. A series of HA-VE macromers with tunable degrees of substitution were synthesized by lipasecatalyzed transesterification. HA-VE gels were proved to be injectable, photocurable, enzymatically degradable and mechanically comparable to various soft tissues. Owing to the unique molecular design, degradation products of HA-VE gels through hydrolysis are non-toxic polyvinyl alcohol and adipic acid. Furthermore, HA-VE gels were systematically characterized and compared to HA-acrylates (HA-AC) and HA-methacrylates (HA-MA) gels including macromer cytotoxicity, photoreactivity, swelling, and gel stiffness. Cytotoxicity assay with L929 fibroblasts revealed that HA-VE was significantly less toxic than HA-AC (P<0.01) and HA-MA (P<0.05). Crosslinking efficiency of HA-VE was comparable to HA-AC and much higher than HA-MA. Although the reactivity of HA-VE for homopolymerization was insufficient for 2PP, it was demonstrated that thiol-ene chemistry could substantially improve its reactivity. This optimization led to 2PP-fabrication of a HA-VE hydrogel construct with m-scale accuracy. Low cytotoxicity, high reactivity and good biodegradability makes HA-VE promising candidates for biological applications in cell culture and tissue engineering.