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Title
Soft sensor for monitoring biomass subpopulations in mammalian cell culture processes
AuthorKroll, Paul ; Stelzer, Ines V. ; Herwig, Christoph
Published in
Biotechnology Letters, 2017, Vol. 39, Issue 11, page 1667-1673
PublishedSpringer Netherlands, 2017
LanguageEnglish
Document typeJournal Article
Keywords (EN)Biomass segregation / Lysed cells / Mammalian cell culture / Monitoring / Soft sensor / Turbidity measurement
ISSN1573-6776
URNurn:nbn:at:at-ubtuw:3-4356 Persistent Identifier (URN)
DOI10.1007/s10529-017-2408-0 
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 The work is publicly available
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Abstract (English)

Objectives

Biomass subpopulations in mammalian cell culture processes cause impurities and influence productivity, which requires this critical process parameter to be monitored in real-time.

Results

For this reason, a novel soft sensor concept for estimating viable, dead and lysed cell concentration was developed, based on the robust and cheap in situ measurements of permittivity and turbidity in combination with a simple model. It could be shown that the turbidity measurements contain information about all investigated biomass subpopulations. The novelty of the developed soft sensor is the real-time estimation of lysed cell concentration, which is directly correlated to process-related impurities such as DNA and host cell protein in the supernatant. Based on data generated by two fed-batch processes the developed soft sensor is described and discussed.

Conclusions

The presented soft sensor concept provides a tool for viable, dead and lysed cell concentration estimation in real-time with adequate accuracy and enables further applications with respect to process optimization and control.

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CC-BY-License (4.0)Creative Commons Attribution 4.0 International License