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Tunable recombinant protein expression in E. coli: promoter systems and genetic constraints
AuthorMarschall, Lukas ; Sagmeister, Patrick ; Herwig, Christoph
Published in
Applied Microbiology and Biotechnology, 2017, Vol. 101, Issue 2, page 501-512
PublishedSpringer Nature, 2017
Document typeJournal Article
Keywords (EN)All-or-none induction / E. coli / Promoter / Transcription / Tunable
URNurn:nbn:at:at-ubtuw:3-3999 Persistent Identifier (URN)
 The work is publicly available
Tunable recombinant protein expression in E. coli: promoter systems and genetic constraints [0.53 mb]
Abstract (English)

Tuning of transcription is a promising strategy to overcome challenges associated with a non-suitable expression rate like outgrowth of segregants, inclusion body formation, metabolic burden and inefficient translocation. By adjusting the expression rateeven on lineto purposeful levels higher product titres and more cost-efficient production processes can be achieved by enabling culture long-term stability and constant product quality. Some tunable systems are registered for patents or already commercially available. Within this contribution, we discuss the induction mechanisms of various Escherichia coli inherent promoter systems with respect to their tunability and review studies using these systems for expression tuning. According to the current level of knowledge, some promoter systems were successfully used for expression tuning, and in some cases, analytical evidence on single-cell level is still pending. However, only a few studies using tunable strains apply a suitable process control strategy. So far, expression tuning has only gathered little attention, but we anticipate that expression tuning harbours great potential for enabling and optimizing the production of a broad spectrum of products in E. coli.

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