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Titel
Self-aliquoting micro-grooves in combination with laser ablation-ICP-mass spectrometry for the analysis of challenging liquids: quantification of lead in whole blood
VerfasserLimbeck, Andreas In der Gemeinsamen Normdatei der DNB nachschlagen ; Nischkauer, Winfried ; Vanhaecke, Frank
Erschienen in
Analytical and Bioanalytical Chemistry, Berlin ; Heidelberg, 2016, Jg. 408, H. 21, S. 5671-5676
ErschienenSpringer, 2016
Ausgabe
Published version
SpracheEnglisch
DokumenttypAufsatz in einer Zeitschrift
Schlagwörter (EN)Laser ablation-ICP-MS / Whole blood analysis / Lead quantification / Dried-droplet laser ablation / Transient signals
ISSN1618-2650
URNurn:nbn:at:at-ubtuw:3-1935 Persistent Identifier (URN)
DOI10.1007/s00216-016-9717-3 
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Self-aliquoting micro-grooves in combination with laser ablation-ICP-mass spectrometry for the analysis of challenging liquids: quantification of lead in whole blood [0.33 mb]
Supplementary material [0.24 mb]
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Zusammenfassung (Englisch)

We present a technique for the fast screening of the lead concentration in whole blood samples using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). The whole blood sample is deposited on a polymeric surface and wiped across a set of micro-grooves previously engraved into the surface. The engraving of the micro-grooves was accomplished with the same laser system used for LA-ICP-MS analysis. In each groove, a part of the liquid blood is trapped, and thus, the sample is divided into sub-aliquots. These aliquots dry quasi instantly and are then investigated by means of LA-ICP-MS. For quantification, external calibration against aqueous standard solutions was relied on, with iron as an internal standard to account for varying volumes of the sample aliquots. The 208Pb/57Fe nuclide ratio used for quantification was obtained via a data treatment protocol so far only used in the context of isotope ratio determination involving transient signals. The method presented here was shown to provide reliable results for Recipe ClinChek® Whole Blood Control levels IIII (nos. 88408842), with a repeatability of typically 3 % relative standard deviation (n=6, for Pb at 442 g L1). Spiked and non-spiked real whole blood was analysed as well, and the results were compared with those obtained via dilution and sectorfield ICP-MS. A good agreement between both methods was observed. The detection limit (3 s) for lead in whole blood was established to be 10 g L1 for the laser ablation method presented here.

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CC-BY-Lizenz (4.0)Creative Commons Namensnennung 4.0 International Lizenz