Within this thesis a method for the in situ monitoring of the adsorption of proteins from aqueous solutions into mesoporous silica supports, was established. Therefore, mesoporous silica films were coated onto ATR crystals for their later implementation in ATR FTIR spectroscopy. Films were prepared using a sol-gel process in combination with an evaporation induced self-assembly approach. Here the formation of mesoporous films with a thickness of 600 nm and 15 nm in pore size using Pluronic F127 as surfactant was accomplished. The proteins Lysozyme and Lipase from Candida rugosa were used for performing adsorption experiments on prepared films monitored by ATR FTIR spectroscopy. The preparation of a stable film and the application of theoretical basics of ATR spectroscopy allowed to ascertain the protein being inside the pores. Lipase from Candida rugosa showed adsorption inside the methylated mesoporous silica film with 15 nm in pore size. Thereby, low concentrations of Lipase in buffer solution (pH 7) ranging from 0.025 - 0.5 mg/mL were detectable. First approaches showed a linear correlation between obtained absorbance and applied concentration. Furthermore, attempts towards monitoring the activity of lipase inside the porous structure were performed. Hence, throughout the preparation of a stable mesoporous silica films on an ATR crystal the interaction of proteins with mesoporous silica can be followed by FTIR spectroscopy. Thereby, first steps towards a potential detection method for low concentrations of proteins in aqueous media were accomplished.