The Danube Region is a key area in the development of Europe. Throughout history, the Danubian countries have been separated and united several times by political changes. Today, the Danube Region connects the most diverse cultures and languages and is home to around 80 million people. As a result of the separation of West and East, both parts of Europe have developed in different ways economically and in terms of infrastructure. Since the enlargement of the European Union to the East, attempts have been made to balance these disparities in the Danube region with EU regional policy and the implementation of the Danube Region Strategy. But the idea of a common development of the Danube region is not shared always. Critics of this approach believe that the countries in the Danube Region do not connect much more except the long river that flows through them. Above all, the Danube Region Strategy is an instrument for obtaining faster access to EU funds. The diploma thesis shows that the Danube region not only brings together the Danube as a common similarity, but that its regions have to face common issues and challenges in their development. These challenges can often only be solved in a collaborative way. The aim of the work is therefore to identify potentials, challenges and problems in the development of regions along the Danube and in the entire Danube region and to elaborate strategic approaches to face them.