Titelaufnahme

Titel
Investigation of light-inducible PcpeC in Nostoc punctiforme ATCC 29133 / von Robin Hoheneder
VerfasserHoheneder, Robin
Begutachter / BegutachterinMach, Robert
ErschienenWien, 2017
Umfang60 Seiten
HochschulschriftTechnische Universität Wien, Diplomarbeit, 2017
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SpracheEnglisch
DokumenttypDiplomarbeit
Schlagwörter (EN)Nostoc punctiforme / Cyanobacteria / light-inducible promoter
URNurn:nbn:at:at-ubtuw:1-104166 Persistent Identifier (URN)
Zugriffsbeschränkung
 Das Werk ist frei verfügbar
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Investigation of light-inducible PcpeC in Nostoc punctiforme ATCC 29133 [2.09 mb]
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Zusammenfassung (Englisch)

The filamentous cyanobacterium Nostoc puntiforme ATCC 29133 is a promising strain for sustainable production of different compounds. The aim of this thesis is to investigate the native light-inducible promoter PcpeC as a tool for future biotechnological approaches. For this work the expression levels of different genes of Synechocystis sp. PCC 6803 and Clamydomonas reinhardtii under the regulation of PcpeC were analyzed. The genes were fused to fluorescence protein YFP or GFP to quantify the protein amount by determination of fluorescence. Three N. punctiforme strains containing the different constructs were tested under different green light conditions for induction, and under red light as repressing conditions. The results showed that there are different expression levels as well as different growth behavior of the tested strains. Furthermore, we were able to show that PcpeC in N. punctiforme ATCC 29133 is regulated by different intensities of green light and that this promoter is completely repressed by red light. Thus indicating its usefulness as a tool to regulate expression of recombinant proteins in cyanobacteria.