Abstract In the field of biomedical application, the generation and design of polymer brushes became very attractive due to their ability to control a number of important architectural features1 for the creation of particular biointerfaces2-4 and applications in nanotechnologies4-6. In the course of this thesis, a strategy for a polymer linker system on glass substrates for T-cell activation was established, in form of end-group functionalized polymers designed for the coupling to biomolecules. For the implementation of this polymer brush system, surface-induced reversible addition fragmentation chain transfer (RAFT) polymerization was selected, delivering gentle polymerization conditions, absence of toxic catalysts, a variety of possible end-group modifications, uniform chain lengths and defined molecular weights. Acryloylmorpholine (NAM)7 and methoxypropylacrylamide (MPAA)8 were chosen as biocompatible monomers. Kinetic studies were performed on the RAFT polymerization of these monomers using 4-cyano-4-(((dodecylthio) carbonothioyl)thio)pentanoic acid (CDTPA) and 2-(((dodecylthio)carbonothioyl)thio)-2-methylpropanoic acid (DDMAT) as RAFT reagents, which were synthesized in the first step according to literature. Both RAFT reagents showed high monomer conversions within short polymerization times. The generated polymers pNAM and pMPAA were investigated on their morphology in aqueous environment with light scattering methods and small angle X-ray scattering, to predict their arrangement in a polymer brush system. It was found out that both polymers formed worm-like shaped aggregates in water and PBS buffer solution. Studies on end-group modification of pNAM and pMPAA polymers were performed by aminolysis and radical induced end-group formation. It proved, that radical induced end-group formation with 4,4'-azobis(4-cyanovaleric acid) performed in good yields and was easy to establish. For the grafting of the polymers from glass substrates by surface immobilized RAFT reagents, further studies were performed. Two approaches were tested: First, to immobilize aminopropyl triethoxysilane (APTES) on the glass surface and then couple the RAFT reagent onto it, second, to immobilize APTES modified RAFT reagents to the surface. These modifications were monitored by contact angle (CA) measurement, total internal reflection fluorescence microscopy (TIRFM), ellipsometry and X-ray photoelectron spectroscopy (XPS). With knowledge gained from these functionalization experiments, a future system for surface induced RAFT polymerization of a suitable polymer brush system will be possible.