In a landmark series of physico-chemical experiments published over some 25 years (1979-2003), Lees and co-workers determined the mineral, organic, and water content of cortical bone samples from all over the vertebrate kingdom, across species ranging from fish to large mammals, from rips to ear bones, and from young to old individuals; thereby elucidating the large compositional variations throughout all these dierent bone tissues. In similarly pioneering neutron diraction and transmission electrone microscopic studies, they also provided satisfying answers on as to how the elementary structural components of bone, namely collagen (making up 90% of the organic matter) and hydroxyapatite mineral, are organized in the extracellular spaces of bone, whereas the question on how the water (with 10% non-collagenous organics within it) is partitioned between the hierarchically organized pore spaces in bone, has remained, up to the knowledge of the authors, largely open. Namely, more recent imaging techniques revealing these fascinating pore morphologies have been never combined with physico-chemical testing of the same samples. This knowledge gap is tackled in the present contribution, by re-viving and refining the original protocols of Lees et al, and combining them with light and scanning electron microscopy. It turns out that handling cortical bone samples on air does not reduce the their water content, so that Lees protocols indeed give access to the entire water in the vascular, lacunar, and ultrastructural pore spaces. In bovine bone, their partition is 13:8:79. The thesis is structured as follows: After a general introduction into the hierarchical structure of bone and the methodology used, Chapter 2 contains a scientific paper summarizing in a concise fashion, the revival and refinement of the Lees protocols in combination with microscopic techniques, providing quantitatively the allocation of bone fluid throughout the dierent pore spaces found within the hierarchical organization of bone. Thereafter, additional technical and methodological aspects are summarized in Chapter 3. Subsequent appendices contain light microscopic images of the surfaces of all tested samples, sketches of the newly developed sample holder, and a conference poster contribution on the topic.