Titelaufnahme

Titel
A bioinformatic approach for detecting genetic mutations leading to multidrug resistance in Fasciola hepatica / by Nina Hofer
Weitere Titel
Ein bioinformatischer Ansatz zur Detektion genetischer Mutationen die zu Multiresistenzen bei Fasciola hepatica führen
VerfasserHofer, Nina
Begutachter / BegutachterinLohninger, Johann
ErschienenWien, 2015
Umfangii, 88 Seiten : Illustrationen
HochschulschriftTechnische Universität Wien, Magisterarbeit, 2015
Anmerkung
Abweichender Titel nach Übersetzung der Verfasserin/des Verfassers
Zusammenfassung in deutscher Sprache
SpracheEnglisch
DokumenttypMasterarbeit
Schlagwörter (DE)Bioinformatik / Mutationen / Genetik
Schlagwörter (EN)bioinformatics / mutations / genetics
URNurn:nbn:at:at-ubtuw:1-80412 Persistent Identifier (URN)
Zugriffsbeschränkung
 Das Werk ist frei verfügbar
Dateien
A bioinformatic approach for detecting genetic mutations leading to multidrug resistance in Fasciola hepatica [4.11 mb]
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Zusammenfassung (Englisch)

This master thesis deals with a bioinformatic approach for detecting genetic mutations leading to multidrug resistance in the trematode Fasciola hepatica. Enhanced anthelmintic drug efflux by ABC transporters has been assumed to be involved in developing resistance. Therefore, cDNA contigs of ABC transporters of drug sensitive versus resistant individuals were analyzed to identify functional significant mutations. The basic local alignment tool (BLAST) was used to compare the Fasciola hepatica contigs with a database consisting of genetic material of Homo sapiens, Drosophila melanogaster and Caenorhabditis elegans. Therefore, the liver fluke contigs could be characterized and uniquely classified to the single ABC transporter subfamilies. In addition, sequence alignments were conducted by the use of Geneious to organize, visualize and compare the classified contigs. Several point mutations could be detected probably leading to functional changes of the transporters. Furthermore, biological motifs were identified and found to be highly degenerate. These results may provide new insights into the adaption mechanisms the parasites have developed to survive drug exposure.