This thesis presents results from a search for the production of supersymmetric particles in proton-proton collisions, using data collected by the CMS experiment during 2011 and 2012. The dataset, recorded during 2011 at a center-of-mass energy of 7 TeV, corresponds to an integrated luminosity of 4.98 fb-1, while in 2012 a dataset of 19.4 fb-1 was recorded at a centre-of-mass energy of 8 TeV. The analysis is performed on events with a single lepton, high hadronic activity, multiple b-quark jets and large missing transverse momentum. The Standard Model background processes are estimated by fitting a model for the spectrum of missing transverse momentum to data in control regions. Signal regions are defined in terms of identified b-quark jet multiplicity and requiring large hadronic activity as well as large missing transverse momentum. No excess with respect to the Standard Model expectation is observed. The results are interpreted in terms of the constrained Minimal Supersymmetric Standard Model and in several simplified models, predicting up to four top quarks from gluino mediated top squark production in the final state. Using the SUSY prediction for the production cross section of gluino-pairs as reference, gluino masses up to 1.2 TeV are excluded for neutralino masses up to 300 GeV, while in case of low mass splittings and a high neutralino mass above 300 GeV, a gluino mass as low as 600 GeV is still allowed.