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Titel
Creating universality for cancer gene therapy in applying Adenovirus vectors with altered tropism : designing Adenovirus particles using K-Coil/E-Coil technology to control receptor specificity / von Birgit Kastberger
VerfasserKastberger, Birgit
Begutachter / BegutachterinGilbert, Rénald ; Kubicek, Peter Christian ; Gadek-Wesierski, Jozefa
Erschienen2011
Umfangii, 91 S. : Ill., graph. Darst.
HochschulschriftWien, Techn. Univ., Dipl.-Arb., 2011
SpracheEnglisch
DokumenttypDiplomarbeit
Schlagwörter (DE)Adenovirus / Gentherapie / K-Coil/E-Coil Technologie / Krebs / Biotechnologie / Tropismus / Car-Rezeptor / GFP-Marker
Schlagwörter (EN)Adenovirus / Gene therapy / K-Coil/E-Coil Technology / Cancer / Biotechnology / Tropism / Car-Receptor / GFP-Marker
URNurn:nbn:at:at-ubtuw:1-42163 Persistent Identifier (URN)
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Creating universality for cancer gene therapy in applying Adenovirus vectors with altered tropism [3.23 mb]
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Zusammenfassung (Englisch)

For my Master Thesis project I was charged with the design of two recombinant types of adenoviruses. The first adenovirus should possess a K-coil sequence situated in the HI-loop region of the fibre's knob domain, the other one was supposed to have an ablated CAR tropism in addition to the identical K-coil insertion. The virus' transduction efficiency via EGF-receptors was monitored by fluorescents provoked by a GFP marker, that had to be inserted into the E1 region of the AdV genome for this reason.