Within the scope of this Ph. D. thesis two types of analytes, namely (glyco)proteins and water-soluble synthetic polymers, were investigated by means of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) and gas phase electrophoretic macromolecular mobility analyzer (GEMMA). The main focal point was the accurate molecular mass determination respectively the determination of the polymeric distribution. Second field of investigation was the analysis of intact fungal spores. Identification and classification of microorganisms, e.g. bacteria and fungi, is of great importance in the field of agriculture, food producing technologies as well as in medical research and diagnosis. Most of the commonly used microbiological techniques are time, cost and labour intensive. Therefore intact cell mass spectrometry (ICMS) via MALDI MS offers a fast and reliable alternative. Mass spectrometric profiles of the analytes present on the surface of the intact microorganism are generated. According to occurring differences in the profiles the microorganisms can be differentiated and identified. For the first time mycotoxin producing Fusarium species common in Austria were analyzed by means of MALDI MS.
Furthermore peptide mass fingerprinting (PMF) conditions were optimized by investigation of proteolytic digestion of (glycol)proteins with MALDI MS. The required sample preparation depends on the instrumental requirements as well as on the properties exhibited by the analyte, thus needs continuous development and optimization.