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<div class="csl-entry">Parvez, S. (2012). <i>Serum auto-antibody testing for early diagnosis of breast cancer</i> [Dissertation, Technische Universität Wien]. reposiTUm. https://resolver.obvsg.at/urn:nbn:at:at-ubtuw:1-56377</div>
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The aim of this thesis is generate prototype-tests suitable for randomized prospective validation of auto-antibody based diagnostic testing using serum samples. Tumours can stimulate the production of auto-antibodies against autologous cellular proteins known as TAAs (tumour associated antigens). This discovery has lead to a possibility of using the auto-antibodies as serological tools for the early diagnosis and management of breast cancer.<br />The recombinant proteins expressed by the SEREX clones, identified from screenings of brain and lung tumour, were used for the production of the protein microarrays and macroarrays. The protein microarrays showed better correlation between the replicates of the serum samples used. The optimized protocols were used for the subsequent experiments. A sizable panel of 642 clone-proteins was selected by marker-screening on protein macroarrays with 38000 clones. These 642 clone-proteins were used to generate protein microarrays that differentiated serum samples from breast cancer patients and controls. Antigenic peptide motifs were identified by in-silico analysis of 642 clone-proteins and peptide arrays were generated using synthetically generated peptides.<br />Comparative studies between protein microarrays and peptide microarrays were done using breast cancer and healthy control samples.<br />Simultaneously, SEREX strategy was used for the identification of the immunogenic TAAs. I identified 192 cDNA expression clones derived from breast cancer tissue samples and the selection was done using breast cancer sera. The genes corresponding to these clones were found over-represented for the pathways that are known to be associated with cancers. These genes showed typical features of TAAs, like over-expression, mutations and fusion genes.
en
dc.language
English
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dc.language.iso
en
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dc.rights.uri
http://rightsstatements.org/vocab/InC/1.0/
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dc.subject
Tumor Autoantikörper
de
dc.subject
Früherkennung
de
dc.subject
Brustkrebs
de
dc.subject
Protein macroarrays
de
dc.subject
Protein microarrays
de
dc.subject
Peptide microarrays
de
dc.subject
SEREX
de
dc.subject
Tumour auto-antibodies
en
dc.subject
early diagnosis
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dc.subject
breast cancer
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dc.subject
Protein macroarrays
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dc.subject
Protein microarrays
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dc.subject
Peptide microarrays
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dc.subject
SEREX
en
dc.title
Serum auto-antibody testing for early diagnosis of breast cancer
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dc.type
Thesis
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dc.type
Hochschulschrift
de
dc.rights.license
In Copyright
en
dc.rights.license
Urheberrechtsschutz
de
dc.contributor.affiliation
TU Wien, Österreich
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dc.rights.holder
Syed Parvez
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tuw.version
vor
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tuw.thesisinformation
Technische Universität Wien
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tuw.publication.orgunit
E166 - Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften