Objectives<br /><br />Biomass subpopulations in mammalian cell culture processes cause impurities and influence productivity, which requires this critical process parameter to be monitored in real-time.<br /><br />Results<br /><br />For this reason, a novel soft sensor concept for estimating viable, dead and lysed cell concentration was developed, based on the robust and cheap in situ measurements of permittivity and turbidity in combination with a simple model. It could be shown that the turbidity measurements contain information about all investigated biomass subpopulations. The novelty of the developed soft sensor is the real-time estimation of lysed cell concentration, which is directly correlated to process-related impurities such as DNA and host cell protein in the supernatant. Based on data generated by two fed-batch processes the developed soft sensor is described and discussed.<br /><br />Conclusions<br /><br />The presented soft sensor concept provides a tool for viable, dead and lysed cell concentration estimation in real-time with adequate accuracy and enables further applications with respect to process optimization and control.
en
dc.language
English
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dc.language.iso
en
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dc.publisher
Springer
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dc.relation.ispartof
Biotechnology Letters
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dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
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dc.subject
Biomass segregation
en
dc.subject
Lysed cells
en
dc.subject
Mammalian cell culture
en
dc.subject
Monitoring
en
dc.subject
Soft sensor
en
dc.subject
Turbidity measurement
en
dc.title
Soft sensor for monitoring biomass subpopulations in mammalian cell culture processes
en
dc.type
Article
en
dc.type
Artikel
de
dc.rights.license
Creative Commons Namensnennung 4.0 International
de
dc.rights.license
Creative Commons Attribution 4.0 International
en
dc.rights.holder
The Author(s) 2017
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dc.type.category
Original Research Article
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tuw.journal.peerreviewed
true
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tuw.peerreviewed
true
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tuw.version
vor
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dcterms.isPartOf.title
Biotechnology Letters
-
tuw.publication.orgunit
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften