<div class="csl-bib-body">
<div class="csl-entry">Hofer, N. (2015). <i>A bioinformatic approach for detecting genetic mutations leading to multidrug resistance in Fasciola hepatica</i> [Master Thesis, Technische Universität Wien]. reposiTUm. https://doi.org/10.34726/hss.2015.34187</div>
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dc.identifier.uri
https://doi.org/10.34726/hss.2015.34187
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/2827
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dc.description
Abweichender Titel nach Übersetzung der Verfasserin/des Verfassers
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dc.description
Zusammenfassung in deutscher Sprache
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dc.description.abstract
This master thesis deals with a bioinformatic approach for detecting genetic mutations leading to multidrug resistance in the trematode Fasciola hepatica. Enhanced anthelmintic drug efflux by ABC transporters has been assumed to be involved in developing resistance. Therefore, cDNA contigs of ABC transporters of drug sensitive versus resistant individuals were analyzed to identify functional significant mutations. The basic local alignment tool (BLAST) was used to compare the Fasciola hepatica contigs with a database consisting of genetic material of Homo sapiens, Drosophila melanogaster and Caenorhabditis elegans. Therefore, the liver fluke contigs could be characterized and uniquely classified to the single ABC transporter subfamilies. In addition, sequence alignments were conducted by the use of Geneious to organize, visualize and compare the classified contigs. Several point mutations could be detected probably leading to functional changes of the transporters. Furthermore, biological motifs were identified and found to be highly degenerate. These results may provide new insights into the adaption mechanisms the parasites have developed to survive drug exposure.
en
dc.language
English
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dc.language.iso
en
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dc.rights.uri
http://rightsstatements.org/vocab/InC/1.0/
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dc.subject
Bioinformatik
de
dc.subject
Mutationen
de
dc.subject
Genetik
de
dc.subject
bioinformatics
en
dc.subject
mutations
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dc.subject
genetics
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dc.title
A bioinformatic approach for detecting genetic mutations leading to multidrug resistance in Fasciola hepatica
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dc.title.alternative
Ein bioinformatischer Ansatz zur Detektion genetischer Mutationen die zu Multiresistenzen bei Fasciola hepatica führen
de
dc.type
Thesis
en
dc.type
Hochschulschrift
de
dc.rights.license
In Copyright
en
dc.rights.license
Urheberrechtsschutz
de
dc.identifier.doi
10.34726/hss.2015.34187
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dc.contributor.affiliation
TU Wien, Österreich
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dc.rights.holder
Nina Hofer
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dc.publisher.place
Wien
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tuw.version
vor
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tuw.thesisinformation
Technische Universität Wien
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tuw.publication.orgunit
E164 - Institut für Chemische Technologien und Analytik