<div class="csl-bib-body">
<div class="csl-entry">Kaineder, A. (2018). <i>High throughput inclusion body sizing and closed loop physiological process control</i> [Diploma Thesis, Technische Universität Wien]. reposiTUm. https://doi.org/10.34726/hss.2018.57270</div>
</div>
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dc.identifier.uri
https://doi.org/10.34726/hss.2018.57270
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/2005
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dc.description.abstract
Quality by design (QbD) became an indispensable part of pharmaceutical quality. To increase product quality, understanding how formulation and manufacturing process variables influence product quality is essential. In that holistic context, this work attempted to develop transferable methods to help increase process understanding, control and robustness. For the purpose of these goals a recombinant protein production process with E. coli as a host was used. The product was formed as inclusion bodies (IB). A high throughput method for IB sizing using nano-particle tracking analysis (NTA) was developed and established. The effect of temperature oscillations on IB solubility could not be investigated as planned due to insufficient heating and cooling performance of the reactor setup. A softsensor, previously developed in the working group, has been further developed and was used for real-time estimation of the specific substrate uptake rate qS. Therefore the obligatory real-time biomass estimation was decoupled from the carbon balance and realized using a weighted average approach, enabling biomass estimation robust against substrate accumulation. Subsequently, the sensor was used to establish a closed loop control of qS. Because of the declining physiological capacity of the host to metabolize substrate (qScrit) during induction phase, a logical query was developed to detect reaching qScrit in real-time. The physiological closed loop control was used to successfully avoid substrate and metabolite accumulation throughout induction phase of an industrial relevant production process.
en
dc.description.abstract
null
de
dc.language
English
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dc.language.iso
en
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dc.rights.uri
http://rightsstatements.org/vocab/InC/1.0/
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dc.subject
physiological process control
en
dc.subject
closed loop control
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dc.subject
physiological capacity
en
dc.subject
inclusion body sizing
en
dc.title
High throughput inclusion body sizing and closed loop physiological process control
en
dc.type
Thesis
en
dc.type
Hochschulschrift
de
dc.rights.license
In Copyright
en
dc.rights.license
Urheberrechtsschutz
de
dc.identifier.doi
10.34726/hss.2018.57270
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dc.contributor.affiliation
TU Wien, Österreich
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dc.rights.holder
Andreas Kaineder
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dc.publisher.place
Wien
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tuw.version
vor
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tuw.thesisinformation
Technische Universität Wien
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tuw.publication.orgunit
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften
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dc.type.qualificationlevel
Diploma
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dc.identifier.libraryid
AC15279113
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dc.description.numberOfPages
63
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dc.identifier.urn
urn:nbn:at:at-ubtuw:1-120990
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dc.thesistype
Diplomarbeit
de
dc.thesistype
Diploma Thesis
en
dc.rights.identifier
In Copyright
en
dc.rights.identifier
Urheberrechtsschutz
de
tuw.advisor.staffStatus
staff
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item.fulltext
with Fulltext
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item.cerifentitytype
Publications
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item.mimetype
application/pdf
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item.openairecristype
http://purl.org/coar/resource_type/c_bdcc
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item.languageiso639-1
en
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item.openaccessfulltext
Open Access
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item.openairetype
master thesis
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item.grantfulltext
open
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crisitem.author.dept
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften