Differential mobility analysis (nES GEMMA instrumentation) applied for virus-like particle research
Victor U. Weiss, Samuele Zoratto, Martina Marchetti-Deschmann
TU Wien, Institute of Chemical Technologies and Analytics, 1060 Vienna, Getreidemarkt 9/164 CTA
Gas-phase electrophoresis applying a nano Electrospray Gas-phase Electrophoretic Mobility Molecular Analyzer (nES GEMMA) instrument is based on an electrospray process. Analytes are transferred from a volatile electrolyte solution to the gas-phase followed by particle drying and charge-equilibration. This latter step results in mostly neutral yet also a considerable amount of single-charged, dry particles. Subsequently, analytes are separated in the nano Differential Mobility Analyzer (nDMA) part of the nES GEMMA by voltage scanning in a tunable electric field and an orthogonal high sheath flow of particle-free air. As particles are singlecharged (neutral species are not regarded), separation of analytes is solely based on their dry particle electrophoretic mobility (EM) diameter. Furthermore, in good accordance with a recommendation of the European Commission for nanoparticle research (2022/C 229/01, June 10th, 2022), particle detection is based on the number of particles – analytes are counted after gas-phase electrophoretic separation yielding a corresponding spectrum comparing EM
diameter and particle count values [1]. nES GEMMA has yielded valuable insights regarding sample purity, analyte stability and aggregational behavior as well as dry particle sizes. Also, given that a correlation based on molecular weight and EM diameter of well-defined standards can be set up, even the molecular weight of an analyte can be assessed from its EM diameter as first demonstrated for proteins [2] and recently even for intact viruses and virus-like particles (VLPs) [3]. Orthogonal analytical techniques like atomic force microscopy (AFM) and mass spectrometry corroborated results obtained from gas-phase electrophoresis. Exemplary results of several VLPs e.g. in development of gene therapy applications will be discussed.
1. Kaufman S.L., et al., Macromolecule analysis based on electrophoretic mobility in air: globular proteins.
Anal Chem, 1996. 68(11): pp. 1895-904.
2. Bacher G., et al., Charge-reduced nano electrospray ionization combined with differential mobility
analysis of peptides, proteins, glycoproteins, noncovalent protein complexes and viruses. J Mass
Spectrom, 2001. 36(9): pp. 1038-52.
3. Weiss V.U., et al., Virus-like particle size and molecular weight/mass determination applying gas-phase
electrophoresis (native nES GEMMA). Anal Bioanal Chem, 2019. 411(23): pp. 5951-62.
We thank Takeda (Vienna, Austria) for providing VLP samples.
en
dc.language.iso
en
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dc.subject
Gas-phase electrophoresis
en
dc.subject
nES GEMMA
en
dc.subject
Virus-like particles
en
dc.subject
VLPs
en
dc.subject
Differential mobility analysis
en
dc.title
Differential mobility analysis (nES GEMMA instrumentation) applied for virus‐like particle research
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.rights.holder
TU Wien
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dc.type.category
Conference Presentation
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tuw.researchTopic.id
M2
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tuw.researchTopic.id
M6
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tuw.researchTopic.id
M4
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tuw.researchTopic.name
Materials Characterization
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.name
Non-metallic Materials
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tuw.researchTopic.value
50
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tuw.researchTopic.value
30
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tuw.researchTopic.value
20
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tuw.publication.orgunit
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
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tuw.author.orcid
0000-0002-0056-6819
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tuw.author.orcid
0000-0002-1491-3404
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tuw.author.orcid
0000-0002-8060-7851
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tuw.event.name
34th MassSpec-Forum Vienna
en
tuw.event.startdate
21-02-2024
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tuw.event.enddate
23-02-2024
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Wien
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tuw.event.country
AT
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tuw.event.institution
Universität Wien
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tuw.event.presenter
Weiss, Victor
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tuw.event.track
Single Track
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wb.sciencebranch
Chemie
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wb.sciencebranch
Medizinische Biotechnologie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.oefos
3040
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wb.sciencebranch.value
80
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wb.sciencebranch.value
20
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item.fulltext
no Fulltext
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item.languageiso639-1
en
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http://purl.org/coar/resource_type/c_18cp
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conference paper not in proceedings
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none
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Publications
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crisitem.author.dept
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
-
crisitem.author.dept
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
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crisitem.author.dept
E164 - Institut für Chemische Technologien und Analytik
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crisitem.author.orcid
0000-0002-0056-6819
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crisitem.author.orcid
0000-0002-1491-3404
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crisitem.author.orcid
0000-0002-8060-7851
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crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
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crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie