In recent years, cancer has been one of the major causes of death. Undoubtedly, the detection and identification of possibly malignant cells in early stages is vital.<br />Infrared (IR) spectroscopy which is based on the absorption of IR light due to molecular vibrations has proven to be able to distinguish mammalian tumour cells from healthy cells. But instead of recording the whole spectrum with a commercial IR spectrometer a distinction can also be achieved by comparing the absorbance of cell samples at only a few specific wavelengths that are characteristic for certain molecules in the cell membrane.<br />In this thesis the design and operation of a cost-effective sensor system for IR absorbance measurements at six wavelengths in the 3.3 - 3.7 µm range is described. The sensor setup consists of IR-LEDs (light emitting diodes), a photodiode detector, and optical components.<br /> CH2 and CH3 of acyl chains in the cell membrane show distinct absorbance peaks at specific wavelengths (symmetric and antisymmetric stretch vibrations). Cell membrane alterations associated with tumour formation result in an absorbance change in the CH2-stretch ratio that can be detected with the sensor system and used for cell type discrimination.<br />IR absorbance measurements were performed on epithelial kidney cell lines (normal MDCK (Madine Darby Canine Kidney) and malignant Caki-1) and melanoma cell lines (M14, A375, and 518A2). Cell samples were measured dried and suspended in PBS (phosphate buffered saline). Hence, a microfluidic chip made of IR transparent calciumflouride comprising a microchamber for cell suspension liquid was developed and realised. Chip design and fabrication steps are presented in this thesis.<br />Measurement results show that a clear and reproducible discrimination between healthy and malignant epithelial kidney cells as well as between the three different melanoma cell lines can be achieved by comparing the CH2- and CH3-stretches.
en
dc.language
English
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dc.language.iso
en
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dc.rights.uri
http://rightsstatements.org/vocab/InC/1.0/
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dc.subject
IR-Absorption
de
dc.subject
Sensorsystem
de
dc.subject
CH2- & CH3-Streckschwingungen
de
dc.subject
Tumorerkennung
de
dc.subject
Mikrofluidik
de
dc.subject
Zellsuspension
de
dc.subject
Nierentumor
de
dc.subject
Melanom
de
dc.subject
IR-absorption
en
dc.subject
sensor system
en
dc.subject
CH2- & CH3-stretches
en
dc.subject
tumour screening
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dc.subject
microfluidics
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dc.subject
suspended cells
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dc.subject
kidney carcinoma
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dc.subject
melanoma
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dc.title
IR-absorption measurements for discrimination of mammalian cells
en
dc.type
Thesis
en
dc.type
Hochschulschrift
de
dc.rights.license
In Copyright
en
dc.rights.license
Urheberrechtsschutz
de
dc.contributor.affiliation
TU Wien, Österreich
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dc.rights.holder
Christoph Haiden
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tuw.version
vor
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tuw.thesisinformation
Technische Universität Wien
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dc.contributor.assistant
Driesche, Sander van den
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tuw.publication.orgunit
E366 - Institut für Sensor- und Aktuatorsysteme
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dc.type.qualificationlevel
Diploma
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dc.identifier.libraryid
AC07809053
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dc.description.numberOfPages
79
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dc.identifier.urn
urn:nbn:at:at-ubtuw:1-42727
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dc.thesistype
Diplomarbeit
de
dc.thesistype
Diploma Thesis
en
dc.rights.identifier
In Copyright
en
dc.rights.identifier
Urheberrechtsschutz
de
item.fulltext
with Fulltext
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item.cerifentitytype
Publications
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item.mimetype
application/pdf
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item.openairecristype
http://purl.org/coar/resource_type/c_bdcc
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item.languageiso639-1
en
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item.openaccessfulltext
Open Access
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item.openairetype
master thesis
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item.grantfulltext
open
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crisitem.author.dept
E366 - Institut für Sensor- und Aktuatorsysteme
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crisitem.author.parentorg
E350 - Fakultät für Elektrotechnik und Informationstechnik